{"id":693,"date":"2026-06-16T10:59:29","date_gmt":"2026-06-16T02:59:29","guid":{"rendered":"https:\/\/www.kz-hub.tech\/?p=693"},"modified":"2026-06-16T11:00:46","modified_gmt":"2026-06-16T03:00:46","slug":"%e4%bd%bf%e7%94%a8ampliconsuite-pipeline%e8%bf%9b%e8%a1%8cecdna%e5%88%86%e6%9e%90","status":"publish","type":"post","link":"https:\/\/www.kz-hub.tech\/index.php\/2026\/06\/16\/%e4%bd%bf%e7%94%a8ampliconsuite-pipeline%e8%bf%9b%e8%a1%8cecdna%e5%88%86%e6%9e%90\/","title":{"rendered":"\u4f7f\u7528AmpliconSuite-pipeline\u8fdb\u884cecDNA\u5206\u6790"},"content":{"rendered":"<p>\u5b98\u65b9github\u7f51\u5740\uff1a<a href=\"https:\/\/github.com\/AmpliconSuite\/AmpliconSuite-pipeline\">https:\/\/github.com\/AmpliconSuite\/AmpliconSuite-pipeline<\/a><br \/>\n\u7533\u8bf7mosek\u4e2a\u4eba\u514d\u8d39\u5b66\u672f\u8bb8\u53ef\uff1a<a href=\"https:\/\/www.mosek.com\/products\/academic-licenses\/\">https:\/\/www.mosek.com\/products\/academic-licenses\/<\/a><br \/>\n\u7533\u8bf7\u540e\u653e\u5728~\/mosek\/\u4e0b<\/p>\n<p>\u5b89\u88c5\u6d41\u7a0b\uff1a<\/p>\n<pre><code>conda create -n ampsuite &amp;&amp; conda activate ampsuite\nconda install -c bioconda -c conda-forge ampliconsuite \nconda install -c mosek mosek\n\n# then run the installer script to finalize the locations of the data repo and mosek license \nwget https:\/\/raw.githubusercontent.com\/AmpliconSuite\/AmpliconSuite-pipeline\/master\/install.sh\nchmod +x install.sh\n.\/install.sh --finalize_only  # -h to see options<\/code><\/pre>\n<p>\u989d\u5916\u81ea\u884c\u4e0b\u8f7d\u6570\u636e\u5e93: <a href=\"https:\/\/refs.ampliconrepository.org\/?prefix=data\/module_support_files\/AmpliconArchitect\/\">https:\/\/refs.ampliconrepository.org\/?prefix=data\/module_support_files\/AmpliconArchitect\/<\/a><br \/>\n\u5df2\u7ecf\u7ecf\u8fc7bwa\u6bd4\u5bf9\uff0c\u4e0b\u8f7d\u6ca1\u6709index\u7684GRCh38.tar.gz\u5373\u53ef<\/p>\n<p>\u5e76\u884c\u811a\u672c\uff1a<\/p>\n<pre><code>AA_DATA_REPO=~\/database\/AmpliconArchitect_repo\/\n\nperl -ne &#039;chomp; next if \/^$\/; @a = split \/\\t\/; print &quot;AmpliconSuite-pipeline.py -s $a[0] -t 4 --normal_bam ..\/align\/$a[1]_bqsr.bam --bam ..\/align\/$a[0]_bqsr.bam --run_AA --run_AC &gt; log\/$a[0].log 2&gt;&amp;1 &amp;&amp; echo $a[0] AmpliconSuite ok\\n&quot;;&#039; ..\/mutect2\/sample_pair.txt &gt; RunAmpliconSuite.sh\n\n# \u8f93\u51fa\u793a\u4f8b\uff1aAmpliconSuite-pipeline.py -s FETB06-BLPT-E -t 4 --normal_bam ..\/align\/FETB06-N_bqsr.bam --bam ..\/align\/FETB06-BLPT-E_bqsr.bam --run_AA --run_AC &gt; log\/FETB06-BLPT-E.log 2&gt;&amp;1 &amp;&amp; echo FETB06-BLPT-E AmpliconSuite ok\n\nnohup bash -c &quot;cat RunAmpliconSuite.sh | parallel -j 4&quot; &gt; log\/AmpliconSuite.log 2&gt;&amp;1 &amp;<\/code><\/pre>\n","protected":false},"excerpt":{"rendered":"<p>\u5b98\u65b9github\u7f51\u5740\uff1ahttps:\/\/github.com\/AmpliconSuite\/AmpliconSui&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-693","post","type-post","status-publish","format-standard","hentry","category-3"],"_links":{"self":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/693","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/comments?post=693"}],"version-history":[{"count":1,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/693\/revisions"}],"predecessor-version":[{"id":694,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/693\/revisions\/694"}],"wp:attachment":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/media?parent=693"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/categories?post=693"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/tags?post=693"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}