{"id":685,"date":"2026-02-09T15:38:55","date_gmt":"2026-02-09T07:38:55","guid":{"rendered":"https:\/\/www.kz-hub.tech\/?p=685"},"modified":"2026-02-09T15:38:55","modified_gmt":"2026-02-09T07:38:55","slug":"%e4%bd%bf%e7%94%a8delly%e8%bf%9b%e8%a1%8c%e7%bb%93%e6%9e%84%e5%8f%98%e5%bc%82%e5%88%86%e6%9e%90","status":"publish","type":"post","link":"https:\/\/www.kz-hub.tech\/index.php\/2026\/02\/09\/%e4%bd%bf%e7%94%a8delly%e8%bf%9b%e8%a1%8c%e7%bb%93%e6%9e%84%e5%8f%98%e5%bc%82%e5%88%86%e6%9e%90\/","title":{"rendered":"\u4f7f\u7528Delly\u8fdb\u884c\u7ed3\u6784\u53d8\u5f02\u5206\u6790"},"content":{"rendered":"<p>github\u5b98\u7f51\uff1a<a href=\"https:\/\/github.com\/dellytools\/delly\">https:\/\/github.com\/dellytools\/delly<\/a><br \/>\nhg38.excl \u4e0b\u8f7d\uff1a<a href=\"https:\/\/github.com\/dellytools\/delly\/tree\/1ac07d0420e8a111076949ac8aab6361eb625e60\/excludeTemplates\">https:\/\/github.com\/dellytools\/delly\/tree\/1ac07d0420e8a111076949ac8aab6361eb625e60\/excludeTemplates<\/a><\/p>\n<h4>Germline short-read SV calling<\/h4>\n<pre><code class=\"language-bash\"># SV calling is done by sample for high-coverage genomes or in small batches for low-coverage genomes\ndelly call -g hg38.fa -o s1.bcf -x hg38.excl sample1.bam\n\n# Merge SV sites into a unified site list\ndelly merge -o sites.bcf s1.bcf s2.bcf ... sN.bcf\n\n# Genotype this merged SV site list across all samples. This can be run in parallel for each sample.\ndelly call -g hg38.fa -v sites.bcf -o s1.geno.bcf -x hg38.excl s1.bam\ndelly call -g hg38.fa -v sites.bcf -o sN.geno.bcf -x hg38.excl sN.bam\n\n# Merge all genotyped samples to get a single VCF\/BCF using bcftools merge\nbcftools merge -m id -O b -o merged.bcf s1.geno.bcf s2.geno.bcf ... sN.geno.bcf\n\n# Apply the germline SV filter which requires at least 20 unrelated samples\ndelly filter -f germline -o germline.bcf merged.bcf\n\n# \u6784\u5efa\u6279\u91cf\u8fd0\u884c\u4ee3\u7801\n<\/code><\/pre>\n<h4>cnv calling<\/h4>\n<pre><code>delly cnv -g example\/ref.fa -m example\/map.fa.gz -c out.cov.gz -o cnv.bcf example\/sr.bam<\/code><\/pre>\n","protected":false},"excerpt":{"rendered":"<p>github\u5b98\u7f51\uff1ahttps:\/\/github.com\/dellytools\/delly hg38.excl &#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-685","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/685","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/comments?post=685"}],"version-history":[{"count":1,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/685\/revisions"}],"predecessor-version":[{"id":686,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/685\/revisions\/686"}],"wp:attachment":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/media?parent=685"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/categories?post=685"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/tags?post=685"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}