{"id":286,"date":"2024-09-11T07:52:19","date_gmt":"2024-09-10T23:52:19","guid":{"rendered":"https:\/\/www.kz-hub.tech\/?p=286"},"modified":"2025-10-24T09:38:58","modified_gmt":"2025-10-24T01:38:58","slug":"bpt-ffpe-%e5%88%87%e7%89%87-he-%e6%9f%93%e8%89%b2%e6%b5%81%e7%a8%8b","status":"publish","type":"post","link":"https:\/\/www.kz-hub.tech\/index.php\/2024\/09\/11\/bpt-ffpe-%e5%88%87%e7%89%87-he-%e6%9f%93%e8%89%b2%e6%b5%81%e7%a8%8b\/","title":{"rendered":"BPT LCM \u7528 FFPE \u5207\u7247 HE \u67d3\u8272\u6d41\u7a0b"},"content":{"rendered":"

0. \u5907\u7269<\/h2>\n
    \n
  1. PEN\u8986\u819c\u73bb\u7247<\/li>\n
  2. \u591a\u805a\u8d56\u6c28\u9178(Poly-L-Lysine, PLL) \u6eb6\u6db2(1x, 0.1mg\/ml ) (\u7ec4\u7ec7\u96be\u9644\u7740\u65f6\u7528\uff0c\u51c0\u6ce1\u73bb\u724724h\uff0c\u5e72\u71e524h)<\/li>\n
  3. Gill's II \u82cf\u6728\u7d20<\/li>\n
  4. \u5206\u5b50\u7b5b<\/li>\n
  5. \u5927\u57f9\u517b\u76bf\uff085\u7247\/\u76bf\uff09<\/li>\n<\/ol>\n

    1. FFPE \u5207\u7247\u8131\u8721+\u68af\u5ea6\u6c34\u5316<\/h2>\n
      \n
    1. \u5207\u7247\u7f6e\u4e8e\u67d3\u8272\u67b6\uff0c\u653e\u516565\u2103\u70d8\u7bb150min<\/li>\n
    2. \u4e8c\u7532\u82efI 15min<\/li>\n
    3. \u4e8c\u7532\u82efII 15min<\/li>\n
    4. \u65e0\u6c34\u4e59\u9187 5min<\/li>\n
    5. 90%\u4e59\u9187 5min<\/li>\n
    6. 80%\u4e59\u9187 5min<\/li>\n
    7. 70%\u4e59\u9187 5min<\/li>\n
    8. \u65e0\u9176\u6c34\u51b2\u6d17 5min<\/li>\n<\/ol>\n
      \u4e8c\u7532\u82ef\u7684\u4f5c\u7528\uff1a\u8131\u53bb\u5207\u7247\u4e2d\u7684\u77f3\u8721\uff0c\u8d77\u900f\u660e\u4f5c\u7528\uff0c\u4f7f\u5207\u7247\u66f4\u5bb9\u6613\u89c2\u5bdf\uff0c\u4f7f\u540e\u9762\u6b65\u9aa4\u67d3\u6db2\u5145\u5206\u8fdb\u5165\u7ec4\u7ec7\u4e2d<\/h6>\n
      \u4e59\u9187\u7684\u4f5c\u7528\uff1a\u6d17\u8131\u4e8c\u7532\u82ef\uff0c\u4f7f\u6c34\u8fdb\u5165\u7ec4\u7ec7\u4e2d\uff0c\u5145\u5206\u6c34\u5316\u3002<\/h6>\n

      2. H(E) \u67d3\u8272<\/h2>\n
        \n
      1. \u82cf\u6728\u7d20\u67d3\u8272
        \n1.1 (\u5f71\u54cdDNA\u63d0\u53d6) Harris\u2019s \u82cf\u6728\u7d20\uff1a\u82cf\u6728\u7d201min--\u81ea\u6765\u6c34\u51b2\u6d175min--\u76d0\u9178\u4e59\u9187\u5206\u53163s--\u81ea\u6765\u6c34\u51b2\u6d1710min\uff08\u8fd4\u84dd\uff09
        \n1.2 Gill\u2019s II \u82cf\u6728\u7d20\uff1a\u82cf\u6728\u7d20\u6ef4\u67d315s\uff0c\u81ea\u6765\u6c34\u51b2\u6d1715min\uff0c\u64e6\u62ed\u8584\u7247\u80cc\u9762\u6b8b\u7559\u82cf\u6728\u7d20<\/li>\n
      2. (\u8df3\u8fc7) \u4f0a\u7ea2\u67d3\u82721min30s\uff0c\u53ef\u752875%\u9152\u7cbe\u8131\u4f0a\u7ea2\uff0c\u6ce8\u610f\u4e5f\u4f1a\u8131\u53bb\u82cf\u6728\u7d20\uff0c\u81ea\u6765\u6c34\u51b2\u6d171min<\/li>\n<\/ol>\n

        3. \u8131\u8721+\u6c34\u5316<\/h2>\n
          \n
        1. \u4e8c\u7532\u82efI 15min<\/li>\n
        2. \u4e8c\u7532\u82efII 15min<\/li>\n
        3. 100%\u4e59\u9187 5min<\/li>\n
        4. 90%\u4e59\u9187 5min<\/li>\n
        5. 80%\u4e59\u9187 5min<\/li>\n
        6. 70%\u4e59\u9187 5min<\/li>\n<\/ol>\n

          4. \u5e72\u71e5+\u50a8\u5b58<\/h2>\n
            \n
          1. \u7f6e\u5165\u52a0\u5165\u4e86\u5206\u5b50\u7b5b\u7684\u76bf\u4e2d2\u5929\u5373\u53ef\u5207\u5272<\/li>\n
          2. \u540e\u7eed\u4fdd\u5b58\u4e0e\u5206\u5b50\u7b5b\u76bf\u4e2d<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

            0. \u5907\u7269 PEN\u8986\u819c\u73bb\u7247 \u591a\u805a\u8d56\u6c28\u9178(Poly-L-Lysine, PLL) \u6eb6\u6db2(1x, 0.1mg\/ml…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-286","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/286","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/comments?post=286"}],"version-history":[{"count":10,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/286\/revisions"}],"predecessor-version":[{"id":645,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/286\/revisions\/645"}],"wp:attachment":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/media?parent=286"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/categories?post=286"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/tags?post=286"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}