{"id":184,"date":"2024-07-18T20:56:17","date_gmt":"2024-07-18T12:56:17","guid":{"rendered":"https:\/\/www.kz-hub.tech\/?p=184"},"modified":"2024-07-18T20:56:17","modified_gmt":"2024-07-18T12:56:17","slug":"vaf%ef%bc%8cmaf%ef%bc%8c%e8%82%bf%e7%98%a4%e7%ba%af%e5%ba%a6%ef%bc%8cmcf%ef%bc%8cccf%e7%9a%84%e6%a6%82%e5%bf%b5%e5%92%8c%e8%ae%a1%e7%ae%97%e6%96%b9%e6%b3%95","status":"publish","type":"post","link":"https:\/\/www.kz-hub.tech\/index.php\/2024\/07\/18\/vaf%ef%bc%8cmaf%ef%bc%8c%e8%82%bf%e7%98%a4%e7%ba%af%e5%ba%a6%ef%bc%8cmcf%ef%bc%8cccf%e7%9a%84%e6%a6%82%e5%bf%b5%e5%92%8c%e8%ae%a1%e7%ae%97%e6%96%b9%e6%b3%95\/","title":{"rendered":"VAF\uff0cMAF\uff0c\u80bf\u7624\u7eaf\u5ea6\uff0cMCF\uff0cCCF\u7684\u6982\u5ff5\u548c\u8ba1\u7b97\u65b9\u6cd5"},"content":{"rendered":"

VAF\u7684\u6982\u5ff5\u548c\u8ba1\u7b97\u65b9\u6cd5<\/h2>\n

VAF\u7684\u5168\u79f0\u662fVariant Allele Frequency\uff08\u53d8\u5f02\u7b49\u4f4d\u57fa\u56e0\u9891\u7387\uff09\u6216Variant Allele Fraction\uff08\u53d8\u5f02\u7b49\u4f4d\u57fa\u56e0\u5206\u6570\uff09\u3002\u7b80\u5355\u6765\u8bf4\u5c31\u662f\u5728\u57fa\u56e0\u7ec4\u67d0\u4e2a\u4f4d\u70b9\u652f\u6301alternate\/mutant allele\u7684reads\u8986\u76d6\u6df1\u5ea6\u5360\u8fd9\u4e2a\u4f4d\u70b9\u603breads\u8986\u76d6\u6df1\u5ea6\u7684\u6bd4\u4f8b\u3002\u4ee5VCF\u6587\u4ef6\u4e2d\u7684\u5b57\u6bb5\u4e3a\u4f8b\uff0c\u5176\u4e2dDP\u4ee3\u8868Total Depth\uff0cAD\u4ee3\u8868Allele Depth\uff0c\u56e0\u6b64VAF\u7684\u8ba1\u7b97\u5c31\u662f\uff1a<\/p>\n

VAF = AlleleDepth\/TotalDepth = AD\/TD<\/code><\/pre>\n
VAF\u7528\u5f97\u6bd4\u8f83\u591a\u7684\u5730\u65b9\u662f\u5728\u4e8c\u500d\u4f53germline\u7684genotyping\u4e2d\uff0c\u6742\u5408\u4f4d\u70b9\u7684VAF\u5728\u9ad8\u6df1\u5ea6\uff08\u6bd4\u5982depth>80\uff09\u60c5\u51b5\u4e0b\u5e94\u8be5\u63a5\u8fd150%\uff1b\u5982\u679cVAF\u63a5\u8fd10.25\/0.75\u8bf4\u660e\u57fa\u56e0\u7ec4\u4e0a\u53ef\u80fd\u8fd8\u6709\u53e6\u4e00\u4efd\u62f7\u8d1d\u3002\u53e6\u4e00\u4e2a\u5e94\u7528\u573a\u666f\u5c31\u662f\u764c\u75c7\u57fa\u56e0\u7ec4\u7684somatic genotyping\u3002\u80bf\u7624\u7ec4\u7ec7\u3001cfDNA\u3001ctDNA\u3001CTC genotyping\u7684\u7ed3\u679c\u4e2d\u4f1a\u5305\u542b\u6b63\u5e38\u7684allele\uff08\u4e0e\u6b63\u5e38\u4f53\u7ec6\u80de\u4e00\u81f4\uff09\u4ee5\u53ca\u7a81\u53d8\u7684allele\uff0c\u5176\u4e2d\u7a81\u53d8allele\u7684\u6240\u5360\u7684\u6bd4\u4f8b\u5c31\u662fVAF\u3002VAF\u53ef\u4ee5\u7528\u4e8e\u63a8\u65ad\u80bf\u7624\u7684\u5f02\u8d28\u6027\u548c\u80bf\u7624\u7eaf\u5ea6\uff0c\u6b64\u5916VAF\u7684\u9ad8\u4f4e\u53ef\u80fd\u4f1a\u5f71\u54cd\u764c\u75c7\u7684\u9884\u540e\u3002<\/p>\n
MAF\u7684\u6982\u5ff5\u548c\u8ba1\u7b97\u65b9\u6cd5<\/h2>\n
MAF\u7684\u5168\u79f0\u662fMinor Allele Frequency\uff08\u6b21\u7b49\u4f4d\u57fa\u56e0\u9891\u7387\uff09\uff0c\u662f\u7fa4\u4f53\u9057\u4f20\u5b66\u4e2d\u7684\u6982\u5ff5\u3002MAF\u63cf\u8ff0\u7684\u662f\u7fa4\u4f53\u4e2d\u4e30\u5ea6\u6b21\u9ad8\uff08\u7b2c\u4e8c\u9ad8\uff09\u7684allele\u7684\u9891\u7387\u3002\u6253\u4e2a\u6bd4\u65b9\uff0c\u57281000\u4e2a\u4eba\u7684\u7fa4\u4f53\u4e2d\uff0c\u67d0\u4e2a\u4f4d\u70b9\u67093\u79cd\u78b1\u57fa\u7c7b\u578b\uff1aA\u3001C\u3001G\u3002\u5176\u4e2dA\u78b1\u57fa\u7684\u9891\u7387\u662f0.6\uff0cC\u78b1\u57fa\u9891\u73870.3\uff0cG\u78b1\u57fa\u9891\u73870.1\uff0c\u90a3\u4e48\u6b21\u7b49\u4f4d\u57fa\u56e0\u9891\u7387\u6307\u7684\u5c31\u662fC\u78b1\u57fa\u7684\u9891\u73870.3\u3002MAF\u53ef\u4ee5\u7528\u6765\u533a\u5206\u67d0\u4e2aallele\u662f\u5e38\u89c1\u7684\u591a\u6001\u6027\u8fd8\u662f\u7a00\u6709\u53d8\u5f02\u3002<\/p>\n
\u8981\u6ce8\u610f\u7684\u662fMAF\u5728\u751f\u7269\u4fe1\u606f\u5b66\u4e2d\u53ef\u80fd\u8fd8\u6307\u4ee3Mutation Annotation Format\u3001Multiple Alignment Format\uff0c\u4e5f\u770b\u5230\u6709\u4eba\u7528mutant allele frequency\u8868\u8ff0VAF\u7684\u6982\u5ff5\uff0c\u6240\u4ee5\u4e00\u5b9a\u8981\u6ce8\u610f\u533a\u5206\u3002<\/p>\n
\u80bf\u7624\u7eaf\u5ea6\u7684\u6982\u5ff5\u548c\u8ba1\u7b97\u65b9\u6cd5<\/h2>\n
\u80bf\u7624\u7eaf\u5ea6\uff08tumor purity\uff09\u6307\u7684\u662f\u6837\u672c\u4e2d\u80bf\u7624\u7ec6\u80de\u5360\u6240\u6709\u7ec6\u80de\u7684\u6bd4\u4f8b\u3002\u56e0\u4e3a\u53d6\u6837\u8fc7\u7a0b\u5f88\u96be\u4fdd\u8bc1\u53d6\u5230\u7684\u6240\u6709\u7ec6\u80de\u90fd\u662f\u80bf\u7624\u7ec6\u80de\uff0c\u5e76\u4e14\u6df7\u6742\u7684\u6b63\u5e38\u4f53\u7ec6\u80de\u4f1a\u5bf9\u540e\u7eed\u7684\u5206\u6790\u4ea7\u751f\u5f71\u54cd\uff08\u6bd4\u5982\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\uff09\uff0c\u56e0\u6b64\u5728\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\u4e2d\u6700\u597d\u8ba1\u7b97\u80bf\u7624\u7eaf\u5ea6\u5e76\u505a\u76f8\u5e94\u7684\u6821\u6b63\u3002\u5e38\u7528\u7684\u4f30\u7b97\u80bf\u7624\u7eaf\u5ea6\u7684\u751f\u7269\u4fe1\u606f\u5b66\u5de5\u5177\u6709\uff1aABSOLUTE\u3001PyClone\u3001SciClone\u3001EXPANDS\u3001ESTIMATE\u7b49\u3002<\/p>\n
MCF\u7684\u6982\u5ff5\u548c\u8ba1\u7b97\u65b9\u6cd5<\/h2>\n
MCF\uff08Mutant Cell Fraction\uff09\u6307\u7684\u662f\u5305\u542b\u67d0\u4e2aSNV\u7684\u7ec6\u80de\u6bd4\u4f8b\u3002\u5728\u7b80\u5316\u6a21\u578b\u4e0b\u2014\u2014\u4e0d\u8003\u8651CNV\uff0c\u5e76\u4e14\u662f\u4e8c\u500d\u4f53\u6742\u5408SNV\u7684\u65f6\u5019\uff0cMCF\u5c31\u662fVAF\u76842\u500d:<\/p>\n
MCF=VAF\u22172<\/code><\/pre>\n
CCF\u7684\u6982\u5ff5\u548c\u8ba1\u7b97\u65b9\u6cd5<\/h2>\n
CCF\uff08Cancer Cell Fraction\uff09\u6307\u7684\u662f\u80bf\u7624\u7ec6\u80de\u4e2d\u5305\u542b\u67d0\u4e2aSNV\u7684\u7ec6\u80de\u6240\u5360\u6bd4\u4f8b\u3002CCF\u548cMCF\u4e4b\u95f4\u4e5f\u53ef\u4ee5\u6362\u7b97\uff0c\u8bbe\u80bf\u7624\u7eaf\u5ea6\u4e3a\u03b1\uff0c\u5728\u4e0d\u8003\u8651CNV\uff0c\u5e76\u4e14\u662f\u4e8c\u500d\u4f53\u6742\u5408SNV\u65f6\uff1a<\/p>\n
CCF = MCF\/\u03b1<\/code><\/pre>\n","protected":false},"excerpt":{"rendered":"
VAF\u7684\u6982\u5ff5\u548c\u8ba1\u7b97\u65b9\u6cd5 VAF\u7684\u5168\u79f0\u662fVariant Allele Frequency\uff08\u53d8\u5f02\u7b49\u4f4d\u57fa\u56e0\u9891\u7387\uff09\u6216V…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-184","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/184","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/comments?post=184"}],"version-history":[{"count":1,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/184\/revisions"}],"predecessor-version":[{"id":185,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/184\/revisions\/185"}],"wp:attachment":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/media?parent=184"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/categories?post=184"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/tags?post=184"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}