{"id":177,"date":"2024-06-27T14:49:17","date_gmt":"2024-06-27T06:49:17","guid":{"rendered":"https:\/\/www.kz-hub.tech\/?p=177"},"modified":"2024-06-27T14:54:15","modified_gmt":"2024-06-27T06:54:15","slug":"%e5%8d%95%e7%bb%86%e8%83%9e%e7%bb%84%e7%bb%87%e8%a7%a3%e7%a6%bb%e6%b5%81%e7%a8%8b","status":"publish","type":"post","link":"https:\/\/www.kz-hub.tech\/index.php\/2024\/06\/27\/%e5%8d%95%e7%bb%86%e8%83%9e%e7%bb%84%e7%bb%87%e8%a7%a3%e7%a6%bb%e6%b5%81%e7%a8%8b\/","title":{"rendered":"\u5355\u7ec6\u80de\u7ec4\u7ec7\u89e3\u79bb\u6d41\u7a0b"},"content":{"rendered":"<h1>\u7ec4\u7ec7\u89e3\u79bb\u6d41\u7a0b<\/h1>\n<h2>\u5907\u7269<\/h2>\n<ol>\n<li>\u6db2\u4f53\uff1aPBS\uff1b1640\uff1b<\/li>\n<li>\u9176\uff1a\u6df7\u5408\u9176\uff1b\u80f0\u9176\uff1b1X\u88c2\u7ea2\u6db2\uff08\u65e0\u9176\u6c34\u7a00\u91ca\uff09\uff1b<\/li>\n<li>\u5de5\u5177\uff1a\u5df4\u6c0f\u7ba1\uff1b\u57f9\u517b\u76bf\uff1b\u51b0\u76d2\uff1b\u6d88\u6bd2\u8fc7\u7684\u526a\u5200\u548c\u954a\u5b50\uff1b\u6ee4\u7f51\uff1b\u79bb\u5fc3\u7ba1\uff1bEP\u7ba1\uff1b\u8ba1\u65f6\u5668\uff1b\u6c34\u6d74\u7bb1\u9884\u70ed37\u2103<\/li>\n<\/ol>\n<h2>\u7b2c\u4e00\u6b65 \u526a\u788e\u7ec4\u7ec7<\/h2>\n<ol>\n<li>\u5c06\u7ec4\u7ec7\u53ca\u4fdd\u5b58\u6db2\uff0cPBS\u5012\u51656cm\u57f9\u517b\u76bf\u4e2d\uff0c\u5254\u9664\u7b4b\u819c\u548c\u8102\u80aa\u7ec4\u7ec7\u540e\uff08\u4e73\u817a\u764c\u53ef\u9002\u5f53\u4fdd\u7559\u8102\u80aa\u7ec4\u7ec7\uff09\uff0c\u7528\u6ce8\u5c04\u5668\u9488\u5934\u5728\u51b0\u4e0a\u5207\u788e\u7ec6\u80de\uff0c\u6700\u7ec8\u5c06\u7ec6\u80de\u788e\u7247\u76f4\u5f841-3mm\uff08\u76f4\u5f84\u5c0f\u4e8e\u5df4\u6c0f\u7ba1\u7684\u7ba1\u53e3\uff09\uff1b<\/li>\n<li>\u7528\u5bbd\u53e3\u67aa\u5934\u5439\u6253\u6df7\u5300\u540e\uff0c\u5c06\u57f9\u517b\u76bf\u4e2d\u7684\u6db2\u4f53\u53ca\u7ec4\u7ec7\u8f6c\u79fb\u81f35ml EP\u7ba1\u4e2d\uff0c\u7528\u7eafPBS\u6e05\u6d17\uff1b\u6bcf\u6b21\u6e05\u6d17\u65f6\u5c06\u7ec4\u7ec7\u5439\u5f00\uff0c\u7136\u540e\u7f6e\u4e8e\u51b0\u4e0a\u81ea\u7136\u6c89\u964d\uff0c\u91cd\u590d2-3\u6b21\u76f4\u81f3\u4e0a\u6e05\u6db2\u6f84\u6e05\u3002<br \/>\n<img decoding=\"async\" src=\"https:\/\/www.kz-hub.tech\/wp-content\/uploads\/2024\/06\/\u5fae\u4fe1\u56fe\u7247_20240627145010-300x169.jpg\" alt=\"\" \/><\/li>\n<\/ol>\n<h2>\u7b2c\u4e8c\u6b65 \u6df7\u5408\u9176\u6d88\u5316<\/h2>\n<ol start=\"3\">\n<li>\u53bb\u4e0a\u6e05\u540e\uff0c\u52a0\u5165\u6df7\u5408\u9176\u540e\u653e\u516537\u2103 125rpm\u6447\u5e8a\uff0c30min\u540e\u89c2\u5bdf\uff0c\u6d88\u5316\u5b8c\u6210\u540e\u7b5b\u7f51\u8fc7\u6ee4\uff0c\u6c89\u6dc0\u79bb\u5fc3\uff08400g\uff0c7min\uff09\uff0c\u5269\u4f59\u7ec4\u7ec7\u884c\u80f0\u9176\u6d88\u5316<\/li>\n<\/ol>\n<h4>\u3010\u4e73\u817a\u764c\u4e13\u7528\u6df7\u5408\u9176\u914d\u65b9\u3011<\/h4>\n<p>\u80f6\u539f\u9176I 0.8mg\/ml<br \/>\n\u80f6\u539f\u9176II 0.8mg\/ml<br \/>\n\u4e2d\u6027\u86cb\u767d\u9176 1mg\/ml<br \/>\n\u900f\u660e\u8d28\u9178\u9176 30u\/ml<br \/>\n1-2% BSA<br \/>\n\u6ce8\uff1a2-5ml EP\u7ba1\uff0c\u52a0\u5165\u9176\u7684\u4f53\u79ef\u7ea6\u7b49\u4e8e\u7ec4\u7ec7\u91cf\u4f53\u79ef\u768410\u500d\u4ee5\u4e0a\uff0c\u82e52-3\u6761\u7ec4\u7ec7\uff0c\u53ef\u52a0\u51651-1.5ml\u6df7\u5408\u9176\uff0c\u653e\u51652mlEP\u7ba1\u5185\u4e0a\u6447\u5e8a\uff0c\u82e5EP\u7ba1\u8fc7\u5927\uff0c\u632f\u52a8\u5e45\u5ea6\u592a\u5927\u4f1a\u9020\u6210\u7ec6\u80de\u6b7b\u4ea1\uff0c\u82e5\u592a\u5c0f\uff0c\u5219\u53c8\u6df7\u5300\u4e0d\u5145\u5206\u3002\u89c2\u5bdf\u65f6\u95f4\uff1a\u95f4\u969410min\uff0c\u4e0a\u6e05\u53d8\u6d51\u6d4a\u540e\u53ef5-8min\u89c2\u5bdf\u3002<br \/>\n\u662f\u5426\u9700\u52a0\u5165\u80f0\u9176\u89c6\u60c5\u51b5\u5b9a\uff0c\u52a0\u5165\u91cf\u540c\u5e38\u89c4\u6bd4\u4f8b\u3002<\/p>\n<h4>\u3010\u76ae\u80a4\u4e13\u7528\u6df7\u5408\u9176\u914d\u65b9\u3011<\/h4>\n<p>\u80f6\u539f\u9176IV 2 mg\/ml<br \/>\n\u4e2d\u6027\u86cb\u767d\u9176 0.8 mg\/ml<br \/>\nDNA\u9176 1 mg\/ml<br \/>\n\u900f\u660e\u8d28\u9178\u9176 50 u\/ml<br \/>\n1-2%BSA<br \/>\n\u526a\u788e\u76ae\u80a4\u7ec4\u7ec7\u540eEDTA\uff0820mM\uff09 37\u2103\u6c34\u6d74\u9707\u836130min<\/p>\n<h2>\u7b2c\u4e09\u6b65 \u80f0\u9176\u6d88\u5316<\/h2>\n<ol start=\"4\">\n<li>\u82e5\u8fd8\u6709\u5269\u4f59\u7ec4\u7ec7\uff0c\u53ef\u52a0\u5165\u80f0\u9176\u884c\u7b2c\u4e8c\u6b21\u6d88\u5316\uff0c\u540c\u683737\u2103 125rpm\u6447\u5e8a\uff0c30min\u540e\u89c2\u5bdf\uff0c\u6d88\u5316\u5b8c\u6210\u540e\u7b5b\u7f51\u8fc7\u6ee4\uff0c\u518d\u6b21\u6c89\u6dc0\u79bb\u5fc3\uff08400g\uff0c7min\uff09\uff0c\u5269\u4f59\u7ec4\u7ec7\u53ef\u4ece\u7b2c\u4e8c\u6b65\u5f00\u59cb\u91cd\u65b0\u6d88\u5316<\/li>\n<\/ol>\n<h2>\u7b2c\u56db\u6b65 \u7ec6\u80de\u60ac\u6db2\u83b7\u53d6<\/h2>\n<p>\u6240\u6709\u6b65\u9aa4\u83b7\u5f97\u7684\u6c89\u6dc0\u75281640\u6216PBS\u91cd\u60ac\u540e\u8ba1\u6570<\/p>\n<h5>\u5355\u7ec6\u80de\u4e0a\u673a\u6807\u51c6\uff1a\u7ec6\u80de\u6d3b\u6027&gt;85%\uff1b\u6709\u6838\u7387&gt;70%\uff1b\u7ed3\u56e2\u7387&lt;20%\u3002\u7ed3\u56e2\u7387\u5982\u8ba4\u4e3a\u4e0d\u53ef\u9760\uff0c\u53ef\u5728\u663e\u5fae\u955c\u4e0b\u8fdb\u4e00\u6b65\u89c2\u5bdf\u3002<\/h5>\n<h2>\u7279\u6b8a\u60c5\u51b5\u5904\u7406<\/h2>\n<h3>\u3010\u6709\u6838\u7387\u4f4e\u2192\u88c2\u7ea2\u3011\uff1a<\/h3>\n<ol>\n<li>\u7ec6\u80de\u60ac\u6db2\u79bb\u5fc3\u540e<\/li>\n<li>\u6c89\u6dc0\u4e2d\u52a0\u51651X\u88c2\u7ea2\u6db21-2ml\uff08\u7ec6\u80de\u4f53\u79ef\u768410\u500d\uff09\uff0c\u51b0\u9762\u4e0a\u88c2\u89e35-6min<\/li>\n<li>\u52a0\u51651-2\u500dPBS\u7ec8\u6b62\u88c2\u7ea2\uff0c\u4e5f\u53ef\u4e0d\u7ec8\u6b62\u76f4\u63a5\u79bb\u5fc3\uff0c\u4f53\u79ef\u8fc7\u5927\u4f1a\u635f\u5931\u8f83\u591a\u7ec6\u80de\u3002<\/li>\n<li>\u79bb\u5fc3300g\uff0c7min\uff0c4\u2103\uff0c\u53bb\u9664\u4e0a\u6e05<\/li>\n<li>1640\u91cd\u60ac\uff085%\uff09\uff0c\u8ba1\u6570\u3002<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>\u7ec4\u7ec7\u89e3\u79bb\u6d41\u7a0b \u5907\u7269 \u6db2\u4f53\uff1aPBS\uff1b1640\uff1b \u9176\uff1a\u6df7\u5408\u9176\uff1b\u80f0\u9176\uff1b1X\u88c2\u7ea2\u6db2\uff08\u65e0\u9176\u6c34\u7a00\u91ca\uff09\uff1b \u5de5\u5177\uff1a\u5df4\u6c0f\u7ba1\uff1b\u57f9\u517b&#8230;<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-177","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/177","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/comments?post=177"}],"version-history":[{"count":2,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/177\/revisions"}],"predecessor-version":[{"id":180,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/posts\/177\/revisions\/180"}],"wp:attachment":[{"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/media?parent=177"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/categories?post=177"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kz-hub.tech\/index.php\/wp-json\/wp\/v2\/tags?post=177"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}